1.  Write 1-2 paragraphs (2/3 page-dbl spaced) describing the work presented. Include in your overview, some background on the topic and the goals and rationale of the study. 

2.  Write 1-2 paragraphs (2/3 page-dbl spaced) describing the experimental design used to address the goal/problem and list the major results from each approach. Be sure to include the two key areas (proteomics results and central metabolism results) and how they connected the results from these two areas to make a complete story. 

3.  Answer the following questions (~1 1/2 page). 

1. Given what you know about regulation of central pathways, why did the authors not identify pathways that were required during infection in their proteomics experiments? 

2. Could an enzyme/pathway be important during infection but not show an effect when it is knocked-out? Why? 
3. What are the main findings in the paper? [hint: what does E. coli eat in the urinary tract?] 

4. What is an auxotroph? How did the author demonstrate auxotrophic phenotypes in vitro? What did the in vivo results from the 2 auxotrophs tell us about the urinary tract environment and how did these data support the main findings of the paper?