1.ADD TO INTRO-What are we trying to accomplish and create through DNA barcoding? DNA barcoding is just a tool we’re using for a larger purpose. How will our research contribute to science and conservation?
- Build a phylogenetic tree- based on the data. Please use this as a road map to complete assignment 3.
You can download MEGA X for free by following this link: https://www.megasoftware.net/
bee samples attached-
Extra info for the tree
You cannot build a MEGA phylogenetic tree out of sequences acquired by different primers. These primers cause different genes to be copied in PCR, so they produce different sequences. MEGA builds a phylogenetic tree by comparing sequences, so adding different sequences from the same specimen would produce a tree telling you that the same specimen is two different species. So you may want to make 2 phylogenetic trees – one for each primer. Comparing the species ID and phylogenetic trees for each primer would be a really interesting discussion topic.
In the interest of creating neat phylogenetic trees, I have attached an excel file with fewer sequences, because there’s no point in creating a phylogenetic tree with several sequences from the same subspecies unless they’re from different locations.
- Results Some kind of table or graph showing bee species (don’t do all of these, just pick 1 or 2 depending on what you would like to show) Add species/subspecies to your pre-existing table that shows concentration and purity (for both primers because different primers give different results)
Or could make a separate table just showing the different species at each site (still use both primers though)
Could make a graph showing the frequency of each subspecies so we can see which are most common 4.Discussion
Interpret your results Which species were common or rare? Were there differences between locations or hives?
Differences between primers?Did one primer tend to produce more bands on the gel? Did one primer produce a different subspecies after sequencing?How does this specifically relate to the larger picture of science and conservation?
Future research directions
NO PLAGIARIZING!!! must be on own words and Every sentence that’s not common knowledge needs a citation at the end of it.